Shotgun sequencing

Applications[ edit ] DNA sequencing may be used to determine the sequence of individual geneslarger genetic regions i.

Shotgun sequencing

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Many programs belong to multiple categories. If you have any suggestions on how to better organize this website - please let us know see below. The goal is to continuously improve the website while not moving too far beyond being a classic link collection and repository of free software for data analysis in mass spectrometry-based proteomics and protein analysis.

The idea is to maximize the usefulness to anyone facing an analytical or computational problem in proteomics who first would like to know if a solution to the problem already exists and is in the public domain. The list will therefore always be an eclectic mix of full software suites with advanced graphical user interfaces, web-based software, libraries, scripts and pieces of source code to accomplish anything from reading a file in a particular format to providing a complete package for a proteomic analysis pipeline, from sample tracking to storing, viewing and comparing results from protein identification search engines.

The ambition should be to maintain only functional links to tested, useful and gratis software. This page was last edited on May 22,at An alternative to whole-genome sequencing is the targeted sequencing of part of a often, this involves just sequencing the protein-coding regions of a genome, which reside within DNA segments called 'exons' and reflect the currently 'best understood' part of most genomes.

Illumina sequencing platforms

DNA sequencing is the process of determining the accurate order of nucleotides along chromosomes and includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA.

The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Compare next-generation sequencing (NGS) platforms, from benchtop to production-scale sequencers, and find the right system for your lab.

Shotgun sequencing

In genetics, shotgun sequencing is a method used for sequencing long DNA strands. It is named by analogy with the rapidly expanding, quasi-random firing pattern of a shotgun.. The chain termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of to base to this size limit, longer sequences are subdivided into smaller fragments that.

Sequencing Glossary & taxonomy Evolving Terminologies for Emerging Technologies Comments?

Software List —

Suggestions Revisions? Mary Chitty MSLS [email protected] Last revised October 30, * Involves performing long-range PCR and sequencing the resulting amplicons (≥ 1 kb). Useful for analyzing long, continuous gene regions.

Ideal for viral and mitochondrial sequencing, HLA analysis, or sequencing only a few targets at a time.

DNA Sequence Assembly | HHMI BioInteractive